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p4 bead spin columns  (Bio-Rad)


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  • 95

    Structured Review

    Bio-Rad p4 bead spin columns
    P4 Bead Spin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p4+bead+spin+columns/Bio-Gel+P-4+Gel/pmc12664897-355-17-21
    Average 95 stars, based on 586 article reviews
    p4 bead spin columns - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus.
    Article Snippet: Next, the aminoddUTP-conjugated DNA oligonucleotides were incubated with 100 μg Cy5-NHS (Sigma-Aldrich, GEPA15101) in 0.1 M HEPES buffer (pH 8.5) for 2 h at room temperature, followed by ethanol precipitation. .. Unlabeled oligonucleotides were removed via high-performance liquid chromatography (HPLC), and the labeled probes were further purified using P4 bead spin columns (Bio-Rad, 1504124). .. Single guide RNAs (sgRNAs) targeting the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions.

    Article Title: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus
    Article Snippet: Next, the amino-ddUTP-conjugated DNA oligonucleotides were incubated with 100 μg Cy5-NHS (Sigma-Aldrich, GEPA15101) in 0.1 M HEPES buffer (pH 8.5) for 2 h at room temperature, followed by ethanol precipitation. .. Unlabeled oligonucleotides were removed via high-performance liquid chromatography (HPLC), and the labeled probes were further purified using P4 bead spin columns (Bio-Rad, 1504124). .. Single guide RNAs (sgRNAs) targeting the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions.

    Labeling:

    Article Title: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus.
    Article Snippet: Next, the aminoddUTP-conjugated DNA oligonucleotides were incubated with 100 μg Cy5-NHS (Sigma-Aldrich, GEPA15101) in 0.1 M HEPES buffer (pH 8.5) for 2 h at room temperature, followed by ethanol precipitation. .. Unlabeled oligonucleotides were removed via high-performance liquid chromatography (HPLC), and the labeled probes were further purified using P4 bead spin columns (Bio-Rad, 1504124). .. Single guide RNAs (sgRNAs) targeting the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions.

    Article Title: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus
    Article Snippet: Next, the amino-ddUTP-conjugated DNA oligonucleotides were incubated with 100 μg Cy5-NHS (Sigma-Aldrich, GEPA15101) in 0.1 M HEPES buffer (pH 8.5) for 2 h at room temperature, followed by ethanol precipitation. .. Unlabeled oligonucleotides were removed via high-performance liquid chromatography (HPLC), and the labeled probes were further purified using P4 bead spin columns (Bio-Rad, 1504124). .. Single guide RNAs (sgRNAs) targeting the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions.

    Purification:

    Article Title: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus.
    Article Snippet: Next, the aminoddUTP-conjugated DNA oligonucleotides were incubated with 100 μg Cy5-NHS (Sigma-Aldrich, GEPA15101) in 0.1 M HEPES buffer (pH 8.5) for 2 h at room temperature, followed by ethanol precipitation. .. Unlabeled oligonucleotides were removed via high-performance liquid chromatography (HPLC), and the labeled probes were further purified using P4 bead spin columns (Bio-Rad, 1504124). .. Single guide RNAs (sgRNAs) targeting the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions.

    Article Title: Imaging the time course of DNA damage response at a nonrepetitive endogenous locus
    Article Snippet: Next, the amino-ddUTP-conjugated DNA oligonucleotides were incubated with 100 μg Cy5-NHS (Sigma-Aldrich, GEPA15101) in 0.1 M HEPES buffer (pH 8.5) for 2 h at room temperature, followed by ethanol precipitation. .. Unlabeled oligonucleotides were removed via high-performance liquid chromatography (HPLC), and the labeled probes were further purified using P4 bead spin columns (Bio-Rad, 1504124). .. Single guide RNAs (sgRNAs) targeting the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions.



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    95
    Bio-Rad p4 bead spin columns
    P4 Bead Spin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p4+bead+spin+columns/Bio-Gel+P-4+Gel/pmc12664897-355-17-21
    Average 95 stars, based on 1 article reviews
    p4 bead spin columns - by Bioz Stars, 2026-10
    95/100 stars
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